E. coli Plasmid Vectors. Methods and Applications

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Biology and other natural sciences - Molecular
E. coli Plasmid Vectors. Methods and...

E. coli Plasmid Vectors. Methods and Applications

Name: E. coli Plasmid Vectors. Methods and Applications
ISBN: 9781592594092

Nicola Casali, Andrew Preston

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A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

Categorías:

Biology and other natural sciences - Molecular

Año:

2003

Edición:

Editorial:

Humana Press

Idioma:

english

Páginas:

305

ISBN 10: